Antioxidant, Cytotoxicity Studies and Isolation of Trans-Anonene and Lupeol from leaves and stem bark extracts of Croton membranaceus

Authors

  • M. S. Isyaka Department of Chemical Sciences, Faculty of Science and Computing, North-Eastern University, Gombe State
  • T., Adamu Department of Chemistry, Faculty of Science, University of Abuja, Gwagwalada, FCT, Abuja
  • A., Umar Department of Chemistry, Faculty of Science, University of Abuja, Gwagwalada, FCT, Abuja
  • B. A. Adedoyin Department of Chemistry, Faculty of Science, University of Abuja, Gwagwalada, FCT, Abuja
  • B. B. Nyakuma Department of Chemical Sciences, Faculty of Science and Computing, North-Eastern University, Gombe State
  • A. A. Sadam Department of Chemistry, Faculty of Science, University of Abuja, Gwagwalada, FCT, Abuja
  • B. M. Mofio Department of Chemistry, Faculty of Science, University of Abuja, Gwagwalada, FCT, Abuja
  • Y. Rufai Chemistry Department, Federal College of Education, Okene, Kogi State, Nigeria
  • J. O. Igoli Department of Chemistry, College of Science, Joseph Sarwuan Tarka University, Makurdi, Benue State, Nigeria
  • S. Jibril Department of Chemical Sciences, Faculty of Science, Federal University of Kashere, Gombe, Nigeria
  • Z. Abdu Department of Chemical Sciences, Faculty of Science, Federal University of Kashere, Gombe, Nigeria

Keywords:

Antioxidant activity, Cytotoxicity, C. membranaceus, Trans-Anonene, Lupeol

Abstract

The antioxidant activity of the dichloromethane (CH2Cl2) and methanol (MeOH) extracts of the

leaves and stem bark of Croton membranaceus was carried out using a 2,2-Diphenyl-1-picryl

hydrazyl (DPPH), 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and metal

chelation assay along with cytotoxicity analysis using the Brine shrimp lethality assay (BSLA).

The silica gel-based chromatographic separation of the CH2Cl2 and MeOH extracts successfully

isolated trans-annonene and lupeol. The structures of the isolated compounds were subsequently

identified using Fourier-transform infrared (FTIR) spectroscopy and nuclear magnetic resonance

(NMR) based on 1D and 2D data compared with the literature. The CH2Cl2 and MeOH extracts

exhibited antioxidant activity in a concentration-dependent manner but were highly cytotoxic. In

conclusion, the study showed that the leaves and stem bark of C. membranaceous are potentially

viable sources of bioactive compounds with potential health and medicinal benefits.

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Published

2024-12-27