Prevalence of Carbapenem Resistant Enterobacteriaceae across One Health Interface from selected Abattoirs in Abeokuta, Ogun State

Abstract

Carbapenem Resistance Enterobacteriaceae (CRE) are commonly detected infectious bacteria in livestock. These pathogens have emerged a global health concern due to their vital roles as vectors for the transfer of resistance genes. This study investigated the prevalence of CRE from selected abattoirs across one health interface. Fifty (50) samples which include; cow beef, soil, water and hand swabs were purposively collected from three major slaughterhouses in Abeokuta Ogun State and assessed phenotypically for CRE after cultural characterization, biochemical and antibiotic susceptibility test. Isolates were confirmed for carbapenemase production using Modified Hodges test (MHT). Four members of Enterobacteriaceae were identified from 56 isolates namely; Escherichia coli (10.7 %), Klebsiella spp. (59 %), Proteus spp. (19.6 %) and Enterobacter spp. (10.7 %). Antibiotic susceptibility test showed that all Bacteria except Enterobacter spp. showed resistance with highest prevalence of 59 % from Klebsiella spp. Further screening showed that 33 out of 46 MDR isolates were carbapenem resistant but confirmatory analysis with MHT proved all CRE negative for carbapenemase production. The study revealed that CRE was significantly high in samples from the abattoir with cattle beef having the highest proportion of Enterobacteriaceae.