Optimization of Fungi Lignocellulose degrading enzymes, isolated from Fadama Soil at Dutse Local Government Area of Jigawa State.

S. I. Mukhtar; S. I. Yahaya; S. Z. Na’inna; N. Baita; Y. S. Abba; M. Raji; H. S. Dauda

Authors

S. I. Mukhtar Department of Microbiology, Federal University, Dutse, Jigawa State.
S. I. Yahaya Department of Microbiology, Bayero University, PMB 3011, Kano State.
S. Z. Na’inna Department of Biological Sciences, Federal University of Kashere PMB 0182, Gombe State
N. Baita Department of Microbiology, Federal University, Dutse, Jigawa State.
Y. S. Abba Department of Microbiology, Federal University, Dutse, Jigawa State.
M. Raji Department of Microbiology, Federal University, Dutse, Jigawa State.
H. S. Dauda Department of Microbiology, Federal University, Dutse, Jigawa State.

Keywords:

optimization, lignocellulose, fadama soil, fungal species

Abstract

This research work was aimed at isolating and identifying fungal species with the potential to produce lignocellulose degrading enzymes and optimizing their culture producing condition. Soil samples were obtained from Madaki, Dadin Duniya and Federal University Dutse from Dutse local government area of Jigawa State. The fungal species isolated from the soil were Aspergillus flavus, Aspergillus fumigatus, Alteneria spp. Mucor spp and Rhizopus spp. The five fungal species were screened for their ability to grow on wood containing agar medium and decolorization of synthetic dye, Remazol Brilliant Blue R (RBBR) and methyl green as an inidicator for the production of lignocellulose degrading enzymes. A. fumigatus was found to have the highest lignocellulose degrading enzymes ability and hence selected for optimization process. The optimum temperature for cellulase activity using wheat bran as substrate was obtained at 45^oC with reducing sugar concentration of 0.47mg/ml with pH of 6 with concentration of 0.50mg/ml at 20 days of incubation with concentration of 0.51mg/ml, 1% substrate concentration of 0.53mg/ml and 10^-3 spore suspension of inoculum size with reducing sugar concentration of 0.49mg/ml. For xylanase, optimization was observed at temperature of 35^oC with reducing sugar concentration of 0.62mg/ml at pH of 6 with concentration of 0.57mg/ml at 20days of incubation using 1% substrate concentration and 10^-3 spore suspension with concentration of 0.58mg/ml and 0.56mg/ml respectively. Finally, the production of lignin degrading enzymes was negligible.